Abstracts · Abstract A2

Liquid Chromatography–mass Spectrometric Assay for the Quantitation in Human Plasma of Nalmefene and Its Application to Pharmacokinetic Studies

Pei Yuan, Lei Lin, Qi Fan, Liangke Zhang

Forensic Science Seminar · 2011 · Volume 1 · Issue 1 · pp. 28

Original Abstract

Abstract

A sensitive, selective and accurate high-performance liquid chromatography-mass spectroscopy (HPLC–MS) assay for the determination of nalmefene in human plasma was developed and validated. The procedure involves protein precipitation and extraction with dichlormethane–hexane mixture. The extracted solutions were then analyzed by a HPLC system, comprising of a shimadzu shim-pack VP-ODS (150mm×2.0mm, 5μm) column and a mobile phase of a mixture of 50mM ammonium acetate and acetonitrile (66:34, v/v), coupled with electrospray ionization mass spectrometry (ESI-MS). The chemical structure analog of nalmefene, Naloxone, was used as the internal standard. The method had a total chromatographic run time of 5.5 min and linear calibration curves over the concentration range of 0.04~15ng/ml. The intra- and inter-assay variations for three different nalmefene levels were less than 10% throughout. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of nalmefene in healthy Chinese volunteers.

Keywords: Nalmefene; Liquid chromatography; Mass spectrometry; Human plasma

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