Forensic Science Seminar
Peer-reviewed forensic science journal · Established 2011
ISSN 2157-118XFOREN SCI SEM · Volume 1

Abstracts · Abstract A2

Liquid Chromatography–mass Spectrometric Assay for the Quantitation in Human Plasma of Nalmefene and Its Application to Pharmacokinetic Studies

Pei Yuan, Lei Lin, Qi Fan, Liangke Zhang

Forensic Science Seminar · 2011 · Volume 1 · Issue 1 · pp. 28

Peer ReviewedISSN 2157-118X Issue date 1 January 2011Public HTML Record
Complete Issue PDF · File A Complete Issue PDF · File B Back to Volume 1, Number 1

Original Abstract

Abstract

A sensitive, selective and accurate high-performance liquid chromatography-mass spectroscopy (HPLC–MS) assay for the determination of nalmefene in human plasma was developed and validated. The procedure involves protein precipitation and extraction with dichlormethane–hexane mixture. The extracted solutions were then analyzed by a HPLC system, comprising of a shimadzu shim-pack VP-ODS (150mm×2.0mm, 5μm) column and a mobile phase of a mixture of 50mM ammonium acetate and acetonitrile (66:34, v/v), coupled with electrospray ionization mass spectrometry (ESI-MS). The chemical structure analog of nalmefene, Naloxone, was used as the internal standard. The method had a total chromatographic run time of 5.5 min and linear calibration curves over the concentration range of 0.04~15ng/ml. The intra- and inter-assay variations for three different nalmefene levels were less than 10% throughout. The method proved to be accurate and specific, and was applied to the pharmacokinetic study of nalmefene in healthy Chinese volunteers.

Keywords: Nalmefene; Liquid chromatography; Mass spectrometry; Human plasma

The original published wording is preserved. No editorial rewriting has been applied.

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Received13 September 2010
Revised30 November 2010
Accepted5 December 2010
Available Online1 January 2011
Source note: The running citation in the supplied source transcription prints “1 (2010) 28”, while the issue, article availability date and archive placement identify this record as Volume 1 Number 1 (2011). The official issue date is used in citation metadata.

Citation Information

Document codeA2 · Abstract
JournalForensic Science Seminar
ISSN2157-118X
Volume / Issue1 / 1
Pages28
Official issue date1 January 2011
Article HTMLhttps://fss.xxyy.info/journal/2011/2157118X.1.A2.html
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