Original Research Papers · Research Paper P1
Determine the Content of Ethanol in Blood by Headspace Gas Chromatography
Original Abstract
Abstract
Objective To establish a rapid detecting method for the determination of ethanol content in blood by headspace gas chromatography (HSGC). Method Blood samples were tested by HSGC with flame ionization detector. Compared with the standard controls operating parallelly, the retention time was used as the qualitative basis. Peak area ratio of ethanol to the internal standard substance was quantified ethanol concentration through the internal standard method. Results Under the optimized conditions of analysis, the coefficient of association was greater than 0.999 within the linearity range 10-300 mg/100 mL. The lowest minimum mass concentration of ethanol in blood was 1 mg/100 mL. The recovery rate was 97.76%-106.55% and the RSD of the degree of precision was less than 2%. Conclusion It was rapid, accurate and highly sensitive that the method could be used to detect ethanol content in blood.
Keywords: Ethanol; Headspace auto-injection; Gas chromatography; Internal standard method; Forensic science
Authors & Publication
Article Details
- Chongqing Engineering Research Center for Criminal Investigation Technology, Institute of Forensic Science, Chongqing Public Security Bureau, Chongqing 400021, China.
- Traffic and Patrol Police Corps, Chongqing Public Security Bureau, Chongqing 400054, China.
Corresponding author: XU Pian · xupian555@126.com
Citation Information
| Document code | P1 · Research Paper |
|---|---|
| Journal | Forensic Science Seminar |
| ISSN | 2157-118X |
| Volume / Issue | 5 / 3 |
| Pages | 113–115 |
| Publication date | 29 December 2015 |
| Article HTML | https://fss.xxyy.info/journal/2015/2157118X.5.3.P1.html |
| Full text PDF | https://fss.xxyy.info/journal/2015/2157118X.5.3.P1.pdf |
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