Original Research Papers · Research Paper P3

Amido Black 10B in the Forensic Investigation - Comparative Studies of Forensic Stain Material

P J Laberke, S Ilg, H-P Bieri, R Hausmann, B Balitzki

Forensic Science Seminar · 2014 · Volume 4 · Issue 1 · pp. 40-44

Original Abstract

Abstract

Bloodstains are among the most meaningful traces in forensic case analysis, which can offer extensive information in forensic case analysis. On the one hand evaluation of the structure, amount, shape and distribution at a crime scene may provide evidence for a reconstruction of events, on the other hand it is now possible to generate a complete DNA profile out of smallest amounts of blood, which can also be used to reconstruct an event and allow conclusions on participation of persons in a crime by molecular genetic analysis. In this study whole blood samples were applied on different substrates and then analyzed for blood using Hexagon OBTI ® . Incubation with two different mixtures of Amido Black 10B showed strong adverse effects and false negative results with the OBTI. In contrast no infl uence of Amido Black 10B was seen in the subsequent DNA analysis.

Keywords: Forensic science; Blood; Indicators and reagents; Sensitivity and specificity; Reliability of results; DNA

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