Original Research Papers · Research Paper P3

Isolation and Quantification of DNA from Blood Samples on Absorbent and Non-absorbent Surfaces

Kaustubh Bhimrao Patil

Forensic Science Seminar · 2013 · Volume 3 · Issue 1 · pp. 67–69

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Abstract

Over the past twenty years, DNA analysis has revolutionized forensic science, and has become a dominant in law enforcement. Today, DNA evidence is a key to the conviction or exoneration of suspects of various types of crime, from theft to rape and murder.DNA is used in forensic laboratories for establishing origin if biological fl uids found at the crime scene. A forensic analyst routinely encounters a variety of challenging biological samples, many of which contain DNA which has been exposed to environmental insults.As blood and blood stains are found at the crime scene, it important to isolate DNA from blood and blood stains. Isolation of DNA from anti-coagulated blood and blood stains were carried out using Bunce method and from clotted blood. Ideally blood sample obtained from suspect or victims should be placed immediately storage but in practice there will always be a deal between collection and sample processing. It is important to determine whether this deal introduces changes into the result obtained from the sample. Therefore, a fi rst aim of this study was to assess the impact, on extracted DNA quality and quantity, from absorbent and non-absorbent surfaces. In the present study it was observed that the collection of blood sample from absorbent and non-absorbent surfaces to isolate the DNA by using Swized method and scratched method. By using PCR method we can detect the quality and quantity of DNA obtained from the absorbent and non-absorbent surfaces. Collection and preservation of various biological materials from crime science thus plays an important role in the isolation of DNA from forensic samples for establishing the link between the crime suspects.

Keywords: Forensic science; Blood; Blood stains; DNA extraction

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